Anti-oxidant activity and protective effects of Lindera obtusiloba flower teas on inflammatory response in Raw 264.7 macrophages induced by lipopolysaccharideopen accessAnti-oxidant activity and protective effects of Lindera obtusiloba flower teas on inflammatory response in Raw 264.7 macrophages induced by lipopolysaccharide
- Other Titles
- Anti-oxidant activity and protective effects of Lindera obtusiloba flower teas on inflammatory response in Raw 264.7 macrophages induced by lipopolysaccharide
- Authors
- Song, Min Chae; Shin, Seung Mi; Seo, Weon Taek; Kim, Hyun Young; Kim, Ji Hyun
- Issue Date
- Mar-2024
- Publisher
- Korean Society for Applied Biological Chemistry
- Keywords
- Flower tea; Free radical; Inflammation; Lindera obtusiloba; Nitric oxide
- Citation
- Journal of Applied Biological Chemistry, v.67, no.1, pp 70 - 77
- Pages
- 8
- Indexed
- SCOPUS
KCI
- Journal Title
- Journal of Applied Biological Chemistry
- Volume
- 67
- Number
- 1
- Start Page
- 70
- End Page
- 77
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/70042
- DOI
- 10.3839/jabc.2024.010
- ISSN
- 1976-0442
2234-7941
- Abstract
- We investigated the in vitro anti-oxidant activity and protective effects of Lindera obtusiloba flower tea extract (LFE) on lipopolysaccharide (LPS)-induced inflammatory response in Raw 264.7 macrophages. The polyphenol and flavonoid contents of LFE were 32.32 mg GAE/g and 213.83 mg QE/g, respectively. The LFE exhibited the highest 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities at a concentration of 100 μg/mL among stem and leaf of Lindera obtusiloba extracts. When treated with LFE at concentrations of 10-100 μg/mL, it dose-dependently reduced the production of nitric oxide (NO) in LPS-induced Raw 264.7 macrophages. The treatment of LFE significantly inhibited inflammatory cytokines such as interleukin (IL)-1β and IL-6 in Raw 264.7 macrophage induced by LPS. In particular, treatment of LFE showed a high suppression of IL-1β expression. To evaluate the anti-inflammatory mechanisms of LFE, we investigated protein expressions such as phospho-nuclear factor kappa-light-chain-enhancer of activated B cells (p-NF-kB), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2). The LFE inhibited the expression of p-NF-kB, iNOS, and COX-2 compared with LPS-treated cells, resulting anti-inflammatory effects of LFE by inhibiting protein expression involved in NF-kB signaling. Therefore, LFE could be a considered as a functional material with anti-oxidant and anti-inflammatory properties. © The Korean Society for Applied Biological Chemistry 2024.
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