Acer okamotoanum inhibits adipocyte differentiation by the regulation of adipogenesis and lipolysis in 3T3-L1 cellsopen access
- Kim, Ji Hyun; Lee, Sanghyun; Kim, Hyun Young; Cho, Eun Ju
- Issue Date
- SPANDIDOS PUBL LTD
- Acer okamotoanum; adipogenesis; AMP kinase; lipolysis; obesity
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.45, no.2, pp.589 - 596
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
- Start Page
- End Page
- Acer okamotoanum is reported to have various antioxidant, anti-inflammatory and beneficial immune system effects. The anti-adipocyte differentiation effects and mechanisms of the ethyl acetate (EtOAc) fraction of an A. okamotoanum extraction was investigated in 3T3-L1 adipocyte cells. Treatment with differentiation inducers increased the level of triglycerides (TGs) in 3T3-L1 adipocyte cells compared with an untreated control. However, the EtOAc fraction of A. okamotoanum significantly decreased TGs. Treatment with 1, 2.5 and 5 mu g/ml showed weak activity, but TG production was inhibited at 10 mu g/ml compared with the control. In addition, A. okamotoanum caused a significant downregulation of proteins related to adipogenesis, such as gamma-cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding protein-alpha, -beta and peroxisome proliferator-activated receptor-gamma, compared with the untreated control. Furthermore, A. okamotoanum significantly upregulated lipolysis related protein, hormone-sensitive lipase and the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK). Therefore, these results indicate that A. okamotoanum suppressed adipogenesis and increased lipolysis and the activation of AMPK, suggesting a protective role in adipocyte differentiation.
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- 자연과학대학 > 식품영양학과 > Journal Articles
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