Insulin secretion and alpha-glucosidase inhibitory effects of dicaffeoylquinic acid derivativesopen access
- Lee, Dahae; Lee, Hak-Dong; Kwon, Hyukjin; Lee, Hye Lim; Hwang, Gwi Seo; Choi, Sungyeol; Kim, Hyun Young; Lee, Sanghyun; Kang, Ki Sung
- Issue Date
- SPRINGER SINGAPORE PTE LTD
- Dicaffeoylquinic acid derivatives; Glucose-stimulated insulin secretion; PDX-1
- APPLIED BIOLOGICAL CHEMISTRY, v.65, no.1
- Journal Title
- APPLIED BIOLOGICAL CHEMISTRY
- In this study, we investigated the effects of dicaffeoylquinic acid derivatives, including 1,4-di-O-caffeoylquinic acid (1,4-DCQA), 3,4-di-O-caffeoylquinic acid (3,4-DCQA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), 4,5-di-O-caffeoylquinic acid (4,5-DCQA), and 1,5-di-O-caffeoylquinic acid (1,5-DCQA) on glucose-stimulated insulin secretion (GSIS) activity and alpha-glucosidase activity were compared in rat INS-1 pancreatic beta-cells. The alpha-glucosidase inhibitory activities of dicaffeoylquinic acid derivatives were as follows: 1,4-DCQA > 1,5-DCQA > 3,4-DCQA > 4,5-DCQA > 3,5-DCQA. In INS-1 cells, dicaffeoylquinic acid derivatives showed no cytotoxic effect at any concentration (2.5-10 mu M). In addition, the GSIS activities of dicaffeoylquinic acid derivatives were as follows: 4,5-DCQA > 3,4-DCQA > 1,4-DCQA > 3,5-DCQA > 1,5-DCQA. Treatment of INS-1 cells with 4,5-DCQA resulted in a marked increase in protein expression of extracellular signal-regulated protein kinases (ERK), insulin receptor substrate-2 (P-IRS-2), Akt, phosphoinositide 3-kinase (P-PI3K), and pancreatic and duodenal homeobox-1 (PDX-1), which might be related to its GSIS activity in INS-1 cells. These findings indicate that the location of the dicaffeoyl functional group influences the anti-diabetic activity of quinic acid.
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- 자연과학대학 > 식품영양학과 > Journal Articles
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