Anti-adipocyte differentiation activity and flavonoid content determination by hplc/uv analysis of tree sprouts
- Kim, J.; Jang, T.; Kim, J.H.; Shin, H.; Park, J.; Lee, S.
- Issue Date
- Korean Society for Applied Biological Chemistry
- Anti-adipocyte differentiation; Flavonoid; HPLC/ UV; Quantitative analysis; Tree sprout
- Journal of Applied Biological Chemistry, v.64, no.3, pp.269 - 275
- Journal Title
- Journal of Applied Biological Chemistry
- Start Page
- End Page
- The in vitro anti-obesity activity of 12 species of tree sprouts in differentiated 3T3-L1 cells and the mechanisms underlying their activity were evaluated. (+)-Catechin and quercetin concentrations in the sprouts were analyzed by HPLC/ UV at 270 and 254 nm, respectively. Euonymus alatus (EAT) and Fraxinus mandschuria (FMS) extracts at doses of 50 and 100 μg/ mL inhibited the accumulation of lipid droplets in differentiated 3T3-L1 cells. Moreover, EAT and FMS downregulated the expression of the CCAAT/enhancer-binding protein-α, adipogenesis-related proteins peroxisome proliferator-activated receptor-γ, and adipocyte P-2α in differentiated 3T3-L1 cells. Tree sprouts with an abundant flavonoid content exerted the highest anti-obesity activity. Concentrations of total flavonoids were the highest in FMS (24.281 mg/g DW) sprouts. These findings could be used to develop health-promoting functional foods or supplements derived from tree sprouts. ? The Korean Society for Applied Biological Chemistry 2021.
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