Free radical scavenging activity of butanol fraction from the fruit of citrus junos

Abstract

In this study, we investigated the free radical [1,1-diphenyl-2- picrylhydrazyl (DPPH), hydroxyl radical (.OH) and superoxide anion (O 2 -)] scavenging activity of MeOH extract and 3 fractions of Citrus junos. Of the tested fractions, the BuOH fraction showed the strongest DPPH scavenging activity, showing the IC50 values of 63.4 mg/mL. Therefore, we continuously carried out DPPH, .OH and O2 - scavenging activity tests of BuOH fraction of Citrus junos. The BuOH fraction of Citrus junos inhibited DPPH radical to 97.5% at a concentration of 1000 mg/mL and the scavenging activities were increased concentration-dependently. In addition, BuOH fraction from Citrus junos also scavenged .OH in a concentration dependent manner from 5 to 1000 mg/mL. Furthermore, BuOH fraction showed about 56% O2 -scavengimg activity at 25 mg/mL concentration but, the scavenging activities were not enhanced in a dose dependent manner. The present results suggest that BuOH fraction of Citrus junos would have the protective potential from oxidative stress induced by free radicals. Copyright ? 2007 KFN.