A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesisopen access
- Authors
- Cha, Joon-Yung; Kim, Woe-Yeon; Kang, Sun Bin; Kim, Jeong Im; Baek, Dongwon; Jung, In Jung; Kim, Mi Ri; Li, Ning; Kim, Hyun-Jin; Nakajima, Masatoshi; Asami, Tadao; Sabir, Jamal S. M.; Park, Hyeong Cheol; Lee, Sang Yeol; Bohnert, Hans J.; Bressan, Ray A.; Pardo, Jose M.; Yun, Dae-Jin
- Issue Date
- Aug-2015
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE COMMUNICATIONS, v.6
- Indexed
- SCIE
SCOPUS
- Journal Title
- NATURE COMMUNICATIONS
- Volume
- 6
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/17089
- DOI
- 10.1038/ncomms9041
- ISSN
- 2041-1723
- Abstract
- YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD-and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD-and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses.
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