Quercetin attenuates neuronal cells damage in a middle cerebral artery occlusion animal modelopen access
- Authors
- Park, Dong-Ju; Shah, Fawad-Ali; Koh, Phil-Ok
- Issue Date
- Apr-2018
- Publisher
- JAPAN SOC VET SCI
- Keywords
- caspase-3; neuroprotection; PARP; quercetin
- Citation
- JOURNAL OF VETERINARY MEDICAL SCIENCE, v.80, no.4, pp.676 - 683
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF VETERINARY MEDICAL SCIENCE
- Volume
- 80
- Number
- 4
- Start Page
- 676
- End Page
- 683
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/11774
- DOI
- 10.1292/jvms.17-0693
- ISSN
- 0916-7250
- Abstract
- Cerebral ischemia is a neurological disorder with high mortality. Quercetin is a flavonoid compound that is abundant in vegetables and fruits. It exerts anti-inflammatory and anti-apoptotic effects. This study investigated the neuroprotective effects of quercetin in focal cerebral ischemia. Male Sprague-Dawley rats were subjected to middle cerebral artery occlusion (MCAO) to induce focal cerebral ischemia. Quercetin or vehicle was injected 30 min before the onset of ischemia. A neurological function test, brain edema measurement, and 2,3,5-triphenyltetrazolium chloride staining were performed to elucidate the neuroprotective effects of quercetin. Western blot analysis was performed to observe caspase-3 and poly ADP-ribose polymerase (PARP) protein expression. MCAO leads to severe neuronal deficits and increases brain edema and infarct volume. However, quercetin administration attenuated the MCAO-induced neuronal deficits and neuronal degeneration. We observed increases in caspase-3 and PARP protein levels in MCAO-operated animals injected with vehicle, whereas quercetin administration attenuated these increases in MCAO injury. This study reveals the neuroprotective effect of quercetin in an MCAO-induced animal model and demonstrates the regulation of caspase-3 and PARP expression by quercetin treatment. These results suggest that quercetin exerts a neuroprotective effect through preventing the MCAO-induced activation of apoptotic pathways affecting caspase-3 and PARP expression.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.gnu.ac.kr/handle/sw.gnu/11774)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.