In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopesopen access
- Authors
- Chowdhury, M. M. R.; Xu, Lianguang; Kong, Rami; Park, Bun-Young; Mesalam, Ayman; Joo, Myeong-Don; Afrin, Fahmida; Jin, Jong-In; Lim, Hyun-Tae; Kong, Il-Keun
- Issue Date
- Jun-2019
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- WholeMom; Sorted sperm; Genetic up-gradation; IVEP
- Citation
- ANIMAL REPRODUCTION SCIENCE, v.205, pp.156 - 164
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANIMAL REPRODUCTION SCIENCE
- Volume
- 205
- Start Page
- 156
- End Page
- 164
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/9115
- DOI
- 10.1016/j.anireprosci.2018.11.006
- ISSN
- 0378-4320
- Abstract
- Sex preselection has always generated great interest among livestock producers. Among the prevalent sperm sorting methods, there is much evidence that sex sorting has a negative effect on sperm quality with an altered pattern of sperm motility, ultimately reducing the period of cell viability. In this study, we have established a new approach for the preselected embryo production by using WholeMom (R); a monoclonal antibody developed against bull sperm epitopes for simple and easy separation of X- and Y-sperm. There were no significant differences (P > 0.05) in the percentage of presumptive zygotes between the control and the X-sperm sorted group, but there was a difference in early cleaving embryos with there being 81.2 +/- 1.4%, 78.3 +/- 1.0%, and 66.7 +/- 1.1% for the control, X-sperm sorted, and Y-sperm sorted groups, respectively. Similarly, the percentage of embryos that developed to the blastocyst stage (Day 7) were also greater (P < 0.05) in the control and X-sperm sorted group compared with the Y-sperm sorted group being 34.8 +/- 1.0%, 32.1 +/- 0.8%, and 23.7 +/- 1.0% in the control, X-sperm sorted, and Y-sperm sorted groups, respectively. Furthermore, B-SRY F2 and B-SRY R2 gene expression data indicated there was a detection accuracy of 81.0% for the female embryos and 72.5% for the male embryos produced in vitro. In conclusion, in cattle in vitro derived embryo production using pre-selected sexed semen and subsequent embryo transfer can facilitate the mass production of individuals that are genetically superior.
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