In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes
DC Field | Value | Language |
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dc.contributor.author | Chowdhury, M. M. R. | - |
dc.contributor.author | Xu, Lianguang | - |
dc.contributor.author | Kong, Rami | - |
dc.contributor.author | Park, Bun-Young | - |
dc.contributor.author | Mesalam, Ayman | - |
dc.contributor.author | Joo, Myeong-Don | - |
dc.contributor.author | Afrin, Fahmida | - |
dc.contributor.author | Jin, Jong-In | - |
dc.contributor.author | Lim, Hyun-Tae | - |
dc.contributor.author | Kong, Il-Keun | - |
dc.date.accessioned | 2022-12-26T14:48:42Z | - |
dc.date.available | 2022-12-26T14:48:42Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 0378-4320 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/9115 | - |
dc.description.abstract | Sex preselection has always generated great interest among livestock producers. Among the prevalent sperm sorting methods, there is much evidence that sex sorting has a negative effect on sperm quality with an altered pattern of sperm motility, ultimately reducing the period of cell viability. In this study, we have established a new approach for the preselected embryo production by using WholeMom (R); a monoclonal antibody developed against bull sperm epitopes for simple and easy separation of X- and Y-sperm. There were no significant differences (P > 0.05) in the percentage of presumptive zygotes between the control and the X-sperm sorted group, but there was a difference in early cleaving embryos with there being 81.2 +/- 1.4%, 78.3 +/- 1.0%, and 66.7 +/- 1.1% for the control, X-sperm sorted, and Y-sperm sorted groups, respectively. Similarly, the percentage of embryos that developed to the blastocyst stage (Day 7) were also greater (P < 0.05) in the control and X-sperm sorted group compared with the Y-sperm sorted group being 34.8 +/- 1.0%, 32.1 +/- 0.8%, and 23.7 +/- 1.0% in the control, X-sperm sorted, and Y-sperm sorted groups, respectively. Furthermore, B-SRY F2 and B-SRY R2 gene expression data indicated there was a detection accuracy of 81.0% for the female embryos and 72.5% for the male embryos produced in vitro. In conclusion, in cattle in vitro derived embryo production using pre-selected sexed semen and subsequent embryo transfer can facilitate the mass production of individuals that are genetically superior. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | BOVINE | - |
dc.subject | FERTILIZATION | - |
dc.subject | SPERMATOZOA | - |
dc.subject | LYCOPENE | - |
dc.subject | QUALITY | - |
dc.title | In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Hyun-Tae | - |
dc.contributor.affiliatedAuthor | Kong, Il-Keun | - |
dc.identifier.doi | 10.1016/j.anireprosci.2018.11.006 | - |
dc.identifier.scopusid | 2-s2.0-85056827447 | - |
dc.identifier.wosid | 000469907300019 | - |
dc.identifier.bibliographicCitation | ANIMAL REPRODUCTION SCIENCE, v.205, pp.156 - 164 | - |
dc.relation.isPartOf | ANIMAL REPRODUCTION SCIENCE | - |
dc.citation.title | ANIMAL REPRODUCTION SCIENCE | - |
dc.citation.volume | 205 | - |
dc.citation.startPage | 156 | - |
dc.citation.endPage | 164 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.subject.keywordPlus | BOVINE | - |
dc.subject.keywordPlus | FERTILIZATION | - |
dc.subject.keywordPlus | SPERMATOZOA | - |
dc.subject.keywordPlus | LYCOPENE | - |
dc.subject.keywordPlus | QUALITY | - |
dc.subject.keywordAuthor | WholeMom | - |
dc.subject.keywordAuthor | Sorted sperm | - |
dc.subject.keywordAuthor | Genetic up-gradation | - |
dc.subject.keywordAuthor | IVEP | - |
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