H2O2및고당유도독성에대한이질풀(Geranium nepalense)추출물의HepG2및HT22세포보호효과

Abstract

In this study, the antioxidant and anti-hyperglycemic effects of Geranium nepalense were investigated in H<inf>2</inf>O<inf>2</inf>- and high glucose-induced oxidative stress models using human hepatic HepG2 and mouse hippocampal HT22 cells. The 20% ethanolic extract of G. nepalense (EGN) revealed potent 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging and markedly inhibited malondialdehyde formation. Antioxidant effects were also observed in 1,1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant power assays. EGN inhibited acetylcholinesterase, advanced glycation end products and α-glucosidase. EGN also increased cell viability and reduced intracellular reactive oxygen species levels, as determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide and 2',7'-dichlorofluorescein diacetate assays. High-performance liquid chromatography analysis identified gallic acid, ellagic acid, and rutin as the major phenolic compounds. © The Korean Society of Food Science and Technology.