LLC-PK1 세포에서 pyrogallol에 의한 산화적 스트레스에 대한 녹차 첨가 서리태 청국장의 보호 효과

Abstract

This study evaluated the antioxidative efficacy of Seoritae Chungkukjang (SC) supplemented with green tea powder (SCG) in an LLC-PK<inf>1</inf> oxidative-stress model. Pyrogallol, a superoxide anion (O<inf>2−</inf> )-generating agent, markedly reduced cell viability, promoted lipid peroxidation, and suppressed antioxidant enzyme activities. Treatment with SC extract counteracted these effects by improving cell viability, inhibiting lipid peroxidation, and enhancing the activities of superoxide dismutase and glutathione peroxidase. Building on this baseline activity, the incorporation of green tea powder further amplified the protective responses, conferring stronger resistance to O<inf>2−</inf>-mediated oxidative injury. Higher levels of green tea powder supplementation produced progressively greater antioxidant benefits. Collectively, these findings indicate that SCG exhibits enhanced antioxidant potential and more effectively mitigated O<inf>2−</inf>-induced oxidative stress in renal epithelial cells compared with SC alone. © The Korean Society for Applied Biological Chemistry 2025.