Effect of <i>Ishige okamurae</i> Extract on Osteoclastogenesis In Vitro and In Vivoopen access
- Authors
- Cho, Su-Hyeon; Kim, Hyun-Soo; Ahn, Juhee; Ryu, Bomi; Jea, Jun-Geon; Lee, Kyubin; Kim, Kyunghwan; Ahn, Ginnae; Lee, Wonwoo; Choi, Kyung-Min; Kim, Kil-Nam
- Issue Date
- Mar-2024
- Publisher
- MDPI
- Keywords
- Ishige okamurae; osteoclasts; NF-kappa B; MAPK; RAW 264.7 cells; zebrafish larvae
- Citation
- MARINE DRUGS, v.22, no.3
- Indexed
- SCIE
SCOPUS
- Journal Title
- MARINE DRUGS
- Volume
- 22
- Number
- 3
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/70219
- DOI
- 10.3390/md22030137
- ISSN
- 1660-3397
1660-3397
- Abstract
- We demonstrated the effect of Ishige okamurae extract (IOE) on the receptor activator of nuclear factor-kappa B ligand (RANKL)-promoted osteoclastogenesis in RAW 264.7 cells and confirmed that IOE inhibited RANKL-induced tartrate-resistant acid phosphatase (TRAP) activity and osteoclast differentiation. IOE inhibited protein expression of TRAP, metallopeptidase-9 (MMP-9), the calcitonin receptor (CTR), and cathepsin K (CTK). IOE treatment suppressed the expression of activated T cell cytoplasmic 1 and activator protein-1, thus controlling the expression of osteoclast-related factors. Moreover, IOE significantly reduced RANKL-phosphorylated extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). It also reduced the RANKL-induced phosphorylation of NF-kappa B and nuclear translocation of p65. IOE inhibited Dex-induced bone loss and osteoclast-related gene expression in zebrafish larvae. HPLC analysis shows that IOE consists of 3.13% and 3.42% DPHC and IPA, respectively. Our results show that IOE has inhibitory effects on osteoclastogenesis in vitro and in vivo and is a potential therapeutic for osteoporosis.
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