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Reversine에 의한 3T3-L1 지방세포의 분화조절Regulation of Adipocyte Differentiation by Reversine in 3T3-L1 Cells

Other Titles
Regulation of Adipocyte Differentiation by Reversine in 3T3-L1 Cells
Authors
김요한최병석문양수
Issue Date
Dec-2023
Publisher
공주대학교 자원과학연구소
Keywords
Adipocyte differentiation; Gene expression; Regulation; Reversine; 3T3-L1
Citation
자원과학연구, v.5, no.2, pp 131 - 141
Pages
11
Indexed
KCICANDI
Journal Title
자원과학연구
Volume
5
Number
2
Start Page
131
End Page
141
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/69609
ISSN
2713-7872
Abstract
Reversine, a synthetic purine analog, is used for cell de-differentiation, and previous research has explored its effects on fat cell de-differentiation and trans-differentiation. However, limited studies have investigated the systematic control of fat cell differentiation during adipogenesis. In this study, we examined the impact of different concentrations and timings of reversine treatments on 3T3-L1 preadipocyte differentiation across various stages, including clonal expansion (day 0 - day 2), post-mitotic growth arrest (day 2 - day 4), and terminal differentiation phases (day 4 - day 6). We found that the differentiation of adipocytes depended on the concentration of reversine, with prolonged treatment leading to inhibited adipocyte differentiation. When treating specific stages with reversine, we observed the most significant decrease in intracellular lipid accumulation (p<0.05) and the expression of adipogenic marker genes (PPARγ, FASN, adiponectin) during the post-mitotic growth arrest phase (p<0.05). The impact of reversine treatment during the late differentiation phase was diminished compared to the two earlier stages of differentiation. However, high concentrations still exhibited inhibitory effects on intracellular lipid accumulation and adipogenic gene expression. Consistent reversine treatment from the early to late stages of differentiation effectively reduced the formation of adipocyte lipid droplets and associated gene expressions (p<0.05), depending on treatment duration and concentration. Our study suggests that reversine generally inhibits adipocyte differentiation, with maximal efficacy during the post-mitotic growth arrest phase.
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농업생명과학대학 (동물생명융합학부)
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