Adenosine receptor Adora2b antagonism attenuates Brucella abortus 544 infection in professional phagocyte RAW 264.7 cells and BALB/c mice
- Authors
- Reyes, Alisha Wehdnesday Bernardo; Vu, Son Hai; Huy, Tran Xuan Ngoc; Min, Wongi; Lee, Hu Jang; Chang, Hong Hee; Lee, John Hwa; Kim, Suk
- Issue Date
- Mar-2020
- Publisher
- ELSEVIER
- Keywords
- Adora2b; Brucella abortus; Cytokines; Macrophages; Spleen
- Citation
- VETERINARY MICROBIOLOGY, v.242
- Indexed
- SCIE
SCOPUS
- Journal Title
- VETERINARY MICROBIOLOGY
- Volume
- 242
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/6862
- DOI
- 10.1016/j.vetmic.2020.108586
- ISSN
- 0378-1135
- Abstract
- Brucella as a stealthy intracellular pathogen avoids activation of innate immune response. Here we investigated the contribution of an adenosine receptor, Adora2b, during Brucella infection in professional phagocyte RAW 264.7 cells and in a murine model. Adora2b-deficient cells showed attenuated Brucella internalization and intracellular survival with enhanced release of IL-6, TNF-alpha, IL-12 and MCP-1. In addition, blockade of Adora2b using MRS 1754 treatment in mice resulted in increased total weight of the spleens but suppressed bacterial burden in these organs accompanied by elevated levels of IL-6, IFN-gamma, TNF-alpha, IL-12 and MCP-1, while reduced IL-10. Overall, we proposed that the Adora2b participates in the successful phagocytic pathway and intracellular survival of Brucella in RAW 264.7 cells, and could be a potential therapeutic target for the treatment of acute brucellosis in animals.
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Collections - 농업생명과학대학 > 축산과학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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