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Reduced NGF Level Promotes Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells Exposed to High Dexamethasone Concentrations

Authors
Hah, Young-SoolYoo, Woong-SunSeo, Seong-WookChung, InyoungKim, Hyun-ACho, Hee YoungKim, Seong-Jae
Issue Date
2-Jun-2020
Publisher
Swets & Zeitlinger
Keywords
Steroid; cataract; nerve growth factor; dexamethasone; epithelial-mesenchymal transition
Citation
Current Eye Research, v.45, no.6, pp 686 - 695
Pages
10
Indexed
SCIE
SCOPUS
Journal Title
Current Eye Research
Volume
45
Number
6
Start Page
686
End Page
695
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/6505
DOI
10.1080/02713683.2019.1695844
ISSN
0271-3683
1460-2202
Abstract
Purpose: To investigate the protective effects of nerve growth factor (NGF) against steroid-induced cataract formation in dexamethasone (Dex)-treated human lens epithelial B-3 (HLE-B3) cells and the possible molecular mechanisms underlying this protection. Materials and Methods: HLE-B3 cells were treated with Dex, and cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay. The levels of expression of NGF, fibronectin, alpha-smooth muscle actin (alpha-SMA), and E-cadherin mRNAs were measured by real-time quantitative polymerase chain reaction (qPCR), and the levels of NGF, fibronectin, alpha-SMA, E-cadherin, tropomyosin receptor kinase A (TrkA), and Akt proteins were measured by Western blot analysis. Gene expression profiles of growth factors in Dex-treated HLE-B3 cells were determined by PCR arrays. In addition, anterior capsule tissue was obtained during cataract surgery, and the specimens were also examined expressions of NGF. Results: NGF was expressed in HLE-B3 cells and also in lens epithelial cells of anterior lens capsules. Dex treatment of HLE-B3 cells increased their expression of epithelial-mesenchymal transition (EMT) markers and migration activity, while markedly downregulating the expression of NGF. NGF treatment significantly reduced the expression of alpha-SMA and fibronectin, as well as cell proliferation. The decreased phosphorylation of p38 MAPK and Akt induced by Dex treatment was significantly reversed by treatment with NGF. Conclusion: NGF/TrkA may repress EMT by targeting the p38 MAPK and pAkt pathways in Dex-treated HLE-B3 cells. NGF may be a novel therapeutic target for patients with steroid-induced cataract.
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