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Reduced NGF Level Promotes Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells Exposed to High Dexamethasone Concentrations

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dc.contributor.authorHah, Young-Sool-
dc.contributor.authorYoo, Woong-Sun-
dc.contributor.authorSeo, Seong-Wook-
dc.contributor.authorChung, Inyoung-
dc.contributor.authorKim, Hyun-A-
dc.contributor.authorCho, Hee Young-
dc.contributor.authorKim, Seong-Jae-
dc.date.accessioned2022-12-26T12:46:08Z-
dc.date.available2022-12-26T12:46:08Z-
dc.date.issued2020-06-02-
dc.identifier.issn0271-3683-
dc.identifier.issn1460-2202-
dc.identifier.urihttps://scholarworks.gnu.ac.kr/handle/sw.gnu/6505-
dc.description.abstractPurpose: To investigate the protective effects of nerve growth factor (NGF) against steroid-induced cataract formation in dexamethasone (Dex)-treated human lens epithelial B-3 (HLE-B3) cells and the possible molecular mechanisms underlying this protection. Materials and Methods: HLE-B3 cells were treated with Dex, and cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay. The levels of expression of NGF, fibronectin, alpha-smooth muscle actin (alpha-SMA), and E-cadherin mRNAs were measured by real-time quantitative polymerase chain reaction (qPCR), and the levels of NGF, fibronectin, alpha-SMA, E-cadherin, tropomyosin receptor kinase A (TrkA), and Akt proteins were measured by Western blot analysis. Gene expression profiles of growth factors in Dex-treated HLE-B3 cells were determined by PCR arrays. In addition, anterior capsule tissue was obtained during cataract surgery, and the specimens were also examined expressions of NGF. Results: NGF was expressed in HLE-B3 cells and also in lens epithelial cells of anterior lens capsules. Dex treatment of HLE-B3 cells increased their expression of epithelial-mesenchymal transition (EMT) markers and migration activity, while markedly downregulating the expression of NGF. NGF treatment significantly reduced the expression of alpha-SMA and fibronectin, as well as cell proliferation. The decreased phosphorylation of p38 MAPK and Akt induced by Dex treatment was significantly reversed by treatment with NGF. Conclusion: NGF/TrkA may repress EMT by targeting the p38 MAPK and pAkt pathways in Dex-treated HLE-B3 cells. NGF may be a novel therapeutic target for patients with steroid-induced cataract.-
dc.format.extent10-
dc.language영어-
dc.language.isoENG-
dc.publisherSwets & Zeitlinger-
dc.titleReduced NGF Level Promotes Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells Exposed to High Dexamethasone Concentrations-
dc.typeArticle-
dc.publisher.location미국-
dc.identifier.doi10.1080/02713683.2019.1695844-
dc.identifier.scopusid2-s2.0-85076442395-
dc.identifier.wosid000533704200006-
dc.identifier.bibliographicCitationCurrent Eye Research, v.45, no.6, pp 686 - 695-
dc.citation.titleCurrent Eye Research-
dc.citation.volume45-
dc.citation.number6-
dc.citation.startPage686-
dc.citation.endPage695-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaOphthalmology-
dc.relation.journalWebOfScienceCategoryOphthalmology-
dc.subject.keywordPlusNERVE GROWTH-FACTOR-
dc.subject.keywordPlusPOSTERIOR CAPSULE OPACIFICATION-
dc.subject.keywordPlusCATARACT-SURGERY-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusASSOCIATION-
dc.subject.keywordPlusBMP-
dc.subject.keywordAuthorSteroid-
dc.subject.keywordAuthorcataract-
dc.subject.keywordAuthornerve growth factor-
dc.subject.keywordAuthordexamethasone-
dc.subject.keywordAuthorepithelial-mesenchymal transition-
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