Decrease of protein phosphatase 2A subunit B by glutamate exposure in the cerebral cortex of neonatal ratsopen access
- Authors
- Kang, Ju-Bin; Park, Dong-Ju; Son, Hyun-Kyoung; Koh, Phil-Ok
- Issue Date
- 18-Sep-2020
- Publisher
- SPRINGERNATURE
- Keywords
- Cerebral cortex; Glutamate; Neonate; PP2A
- Citation
- LABORATORY ANIMAL RESEARCH, v.36, no.1
- Indexed
- KCI
- Journal Title
- LABORATORY ANIMAL RESEARCH
- Volume
- 36
- Number
- 1
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/6178
- DOI
- 10.1186/s42826-020-00064-y
- ISSN
- 1738-6055
- Abstract
- Glutamate induces neurotoxicity during brain development, causing nerve damage. Protein phosphatase 2A (PP2A) is a type of serine/threonine phosphatase that regulates various biological functions. Among the PP2A subunit types, subunit B is abundant in brain tissue and plays an essential role in the nervous system. This study investigated changes in PP2A subunit B expression through glutamate exposure in the cerebral cortex of newborn rats. Sprague-Dawley rat pups (7 days after birth) were injected intraperitoneally with vehicle or glutamate (10 mg/kg). After 4 h of drug treatment, the brain tissue was isolated and fixed for morphological study. In addition, the cerebral cortex was collected for RNA and protein works. We observed severe histopathological changes including swollen neuron and atrophied dendrite in the glutamate exposed cerebral cortex. Glutamate exposure leads to a decrease in PP2A subunit B. Reverse-transcription PCR and Western blot analyses confirmed that glutamate induces a decrease of PP2A subunit B in the cerebral cortex of newborn rats. Moreover, immunohistochemical study showed a decrease in PP2A subunit B positive cells. The reduction of PP2A subunit B expression is considered an indicator of neurodegenerative damage. These results suggest that glutamate exposure causes neuronal damage in the cerebral cortex of new born rats through a decrease in PP2A subunit B.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.