Detailed Information

Cited 7 time in webofscience Cited 6 time in scopus
Metadata Downloads

Erythromycin Treatment of Brassica campestris Seedlings Impacts the Photosynthetic and Protein Synthesis Pathwaysopen access

Authors
Yoon, Young-EunCho, Hyun MinBae, Dong-wonLee, Sung JoongChoe, HyeonjiKim, Min ChulCheong, Mi SunLee, Yong Bok
Issue Date
Dec-2020
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Keywords
antibiotics; erythromycin (Ery); Brassica campestris; proteomics; photosynthesis; protein synthesis; ribosome
Citation
Life, v.10, no.12, pp 1 - 18
Pages
18
Indexed
SCIE
SCOPUS
Journal Title
Life
Volume
10
Number
12
Start Page
1
End Page
18
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/5881
DOI
10.3390/life10120311
ISSN
0024-3019
2075-1729
Abstract
Erythromycin (Ery) is a commonly used veterinary drug that prevents infections and promotes the growth of farm animals. Ery is often detected in agricultural fields due to the effects of manure application in the ecosystem. However, there is a lack of information on Ery toxicity in crops. In this study, we performed a comparative proteomic analysis to identify the molecular mechanisms of Ery toxicity during seedling growth based on our observation of a decrease in chlorophyll (Chl) contents using Brassica campestris. A total of 452 differentially abundant proteins (DAPs) were identified including a ribulose-1,5-bisphosphate carboxylase (RuBisCO). The proteomic analysis according to gene ontology (GO) classification revealed that many of these DAPs responding to Ery treatment functioned in a cellular process and a metabolic process. The molecular function analysis showed that DAPs classified within catalytic activity were predominantly changed by Ery, including metabolite interconversion enzyme and protein modifying enzyme. An analysis of functional pathways using MapMan revealed that many photosynthesis components were downregulated, whereas many protein biosynthesis components were upregulated. A good relationship was observed between protein and transcript abundance in a photosynthetic pathway, as determined by qPCR analysis. These combined results suggest that Ery affects plant physiological activity by downregulating protein abundance in the photosynthetic pathway.
Files in This Item
There are no files associated with this item.
Appears in
Collections
ETC > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Min Chul photo

Kim, Min Chul
대학원 (응용생명과학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE