Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakaiopen access
- Lee, S.-W.; Lee, S.J.; Han, E.-H.; Shin, Y.-W.; Kim, Y.-H.
- Issue Date
- Korean Society of Plant Biotechnology
- Angelica; High-resolution melting; Internal transcribed spacer regions of rDNA; Single nucleotide polymorphisms
- Journal of Plant Biotechnology, v.48, no.2, pp.71 - 76
- Journal Title
- Journal of Plant Biotechnology
- Start Page
- End Page
- Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries. Korean Society for Plant Biotechnology.
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- 사범대학 > 생물교육과 > Journal Articles
- 자연과학대학 > 항노화신소재과학과 > Journal Articles
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