Improved production of rifamycin SV by Amycolatopsis mediterranie MM2 by the feeding method of carbon source

Abstract

The purpose of this research was to study the enhancement of rifamycin SV (RSV) yield according to the feeding method of a carbon source for Amycolatopsis mediterranei MM2, RSV produced during fermentation dropped sharply after reaching a maximum, and it was found that this trend of RSV production resulted from simultaneous production and degradation of RSV. To reduce RSV degradation during incubation, the effect of carbon source on cell growth was investigated. Based on the results, glucose was a better carbon source than glycerol for cell growth, although glycerol was better than glucose for RSV production, as reported in our previous study. To confirm this, RSV yield and dry cell weight (DCW) were measured according to the feeding method of carbon source using a 5-L size bioreactor. Results showed that initial cell growth rate and RSV yield were significantly increased regardless of the addition method of glucose into glycerol, provided glucose was present in the initial stage of cell growth. (C) KSBB.