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Efficient release of ferulic acid from sweet patato (Ipomoea batatas) stems by chemical hydrolysis
- Authors
- Choi, Myung-Suk; Park, Ji-Hyun; Min, Ji Yun; Lim, Bu-Kug; Lee, Byung Hyun; Jung, Gang Won; Lee, Jong-Yoon; Karigar, Chandrakant S.; Yang, Jae-Kyung
- Issue Date
- May-2008
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- ferulic acid; phenolic compounds; sweet potato; acid/base hydrolysis
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.3, pp 319 - 324
- Pages
- 6
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 13
- Number
- 3
- Start Page
- 319
- End Page
- 324
- ISSN
- 1226-8372
1976-3816
- Abstract
- An efficient method for release of ferulic acid from sweet potato stems was developed. Ferulic acid along with phenolic compounds were released from stems by acid and alkaline treatments. The base hydrolysis with 0.1 N NaOH yielded the highest quantity of total extracts (471.1 mg/g). The stems released more phenolic compounds when 0.0125 similar to 0.025 N NaOH was employed. Where as ferulic acid release was maximal with 0.05 N H2SO4 (0.32 mg/g). Ferulic acid was separated from phenolics by column chromatography. Among the elution solvents, ethyl acetate fractions (80%) contained ferulic acid. Ethyl acetate eluants were further fractionated with n-hexane/ethyl acetate/formic acid (100/50/0.5, v/v/v). All fractions showed ferulic acid and phenolic compounds. Fraction V among them was ascribed to ferulic acid with an yield of 5.41 mg/g of dry sweet potato tissue. (C) KSBB.
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