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Heterologous Gene Expression of aprE2 Encoding a 29 kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716
- Authors
- Kwon, Gun-Hee; Jeong, Woo-Ju; Lee, Ae Ran; Park, Jae-Yong; Cha, Jaeho; Song, Young-Sun; Kim, Jeong Hwan
- Issue Date
- Dec-2008
- Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
- Keywords
- Bacillus licheniformis; fibrinolytic enzyme; gene expression; transformation; Bacillus subtilis
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.17, no.6, pp 1372 - 1375
- Pages
- 4
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 17
- Number
- 6
- Start Page
- 1372
- End Page
- 1375
- ISSN
- 1226-7708
2092-6456
- Abstract
- The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using a Bacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantly compared to B. licheniformis 10716 control cell. During the 100 hr incubation in Luria-Bertaini broth at 37 degrees C, fibrinolytic activity of B. licheniformis TF increased rapidly at the late growth stage, after 52 fir of incubation, which was confirmed by zymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media, 60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.
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