Protective effect of enzymatic extracts from sargassum coreanum on h2O2-induced cell damage

Abstract

In our previous study, we preliminarily demonstrated that Celluclast and Neutrase extracts exhibited the strongest H202-scavenging activities among five carbohydrases (Viscozyme, Celluclast, Termamyl, Ultraflo and AMG) and five proteases (Kojizyme, Alcalse, Flavourzyme Protamex and Neutrase) extracts. Thus, Celluclast and Neutrase extracts were selected for use in further experiments and were separated into four different molecular weight fractions (<5, 5-10, 10-30 and >30 kDa). Among them, the 5-10 kDa fraction showed the highest H202-scavenging activity. The 5-10 kDa fraction also strongly enhanced cell viability against H202-induced oxidative damage. Furthermore, the fraction reduced the proportion of apoptotic cells induced by H202, as demonstrated by decreased sub-Gi hypodiploid cells and decreased apoptotic body formation by flow cytometry. These results indicated that the 5-10 kDa fraction of the Celluclast and Neutrase extracts from S. coreanum exhibited strong antioxidant activity over H202-mediated cell damage in vitro.