Effect of Chinese prescription Kangen-karyu on lipid metabolism in type 2 diabetic db/db mice

Abstract

Aim of the study: Chinese prescription Kangen-karyu is clinically used as a treatment for cardiovascular diseases, and we have reported the beneficial effects on chemically induced hyperlipidemic animal models. The present study was investigated to evaluate the hypolipidemic effect of Kangen-karyu in type 2 diabetic db/. db mice which has not been explored yet. Materials and methods: Male db/. db mice were divided into three groups, vehicle (control), Kangen-karyu 100, or 200. mg/kg body weight/day, and orally administered for 5 weeks every day. Age-matched non-diabetic m/. m mice were used as a normal group. Results: Serum triglyceride (TG) and total cholesterol levels in db/. db mice were increased compared with those of m/. m mice. However, the administration of Kangen-karyu reduced hyperlipidemia in db/. db mice through a decline in the serum levels of TG and total cholesterol. The hepatic TG and total cholesterol levels of db/. db mice were markedly higher than those of m/. m mice, but these elevated lipid levels were significantly reduced by 200. mg/kg Kangen-karyu administration. Also, oil red O staining showed that the increased lipid deposition level in the liver of db/. db control mice was improved by Kangen-karyu administration. The expression of sterol regulatory element-binding protein-1 in the liver of db/. db mice was significantly down-regulated by the administration of Kangen-karyu at a dose of 200. mg/kg body weight. Kangen-karyu caused a slight elevation in the expression of peroxisome proliferator-activated receptor α in the liver of db/. db mice. Conclusions: This study provides scientific evidence that Kangen-karyu improves hyperlipidemia and lipid deposition via the regulation of hepatic SREBP-1 expression in type 2 diabetic db/. db mice. ? 2010 Elsevier Ireland Ltd.