Hesperidin과 Hesperetin의 in vitro에서의 항산화 효과

Abstract

In this study, we investigated the antioxidant activity of hesperidin and hesperetin, which are the active compounds from Citrus junos, under in vitro. We measured the scavenging activities of hesperidin and hesperetin on 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical (․OH), nitric oxide (NO) and the inhibition activities on oxidation of protein and lipid under in vitro. Hesperidin and hesperetin dose-dependently scavenged DPPH, ․OH and nitric oxide (NO). In addition, hesperidin and hesperetin significantly reduced oxidation of protein and lipid induced by 2,2'-azobis(2-aminopropane) dihydrochloride. Hesperidin had stronger activity in protein oxidation, on the other hand hesperetin had stronger activity in lipid oxidation. The present study indicates that hesperidin and hesperetin would have antioxidant activities by protecting against oxidative damage induced by free radicals. (Cancer Prev Res 15, 333-339, 2010)