Preparation of Folic Acid-loaded WPI (Whey Protein Isolate) Nanoparticles by Cold-induced Gelation

Abstract

Folate loaded Wl"I (whey protein isolate) nanoparlicles weic cold-incluced gelation piocess Theaml of this work was to investigate the effects ol'process pajanieters. SUCh as the concentration ofthe Will solution, pl-1. temperature. etc. on the ploperties ofnanoparticles The results show that the sinallest nanopi-ticles were obtained when a W131 concentration of' I TO MIS Used it a of 80 (<330 nin) In the c.ise ofthe concentration of C"31 the werc obtained m a concentiation of"5 niM Al-ginate produced the smallest nican size with the narrowest particic sizeclistilbl weie prepared vith k-camweenan As the W-0 ratio Increased. the lileall p'll-ticle size also increased When the release profile was analyzed, the particles were shown to be stable foi nioie than 6 h at a pH of' 1 2, where almost all ofthe folic acid was released within 2 h in the dissolution mcclia of' 1313S at a pl-l of 7 4 Thus. the process paianicterv appear to be important fixtors that affect the properties of nanoparticles