IN VITRO PROPAGATION OF CHAMAECYPARIS OBTUSA SIEB. ET ZUCC.

Abstract

Protocol for in vitro propagation of Chamaecyparis obtusa was established. Adventitious shoots were initiated from epicotyl explants after 4 weeks of culture on MS medium supplemented with 3% (w/v) sucrose and several concentrations of BAP. The effects of various nutritive media, carbon sources and several concentrations (4.44 to 22.20 mu M) of BAP on in vitro shoot (upside of plantlet excepted roots) elongation and multiplication were determined. Optimal shoot growth was achieved on Woody Plant (WP) medium, they have over 25 shoots and 2.5 growth index (GI) after 8 weeks of culture. Among the carbon sources tested, 1% (w/v) fructose showed the highest length of the shoots (appr. 2.2 GI). The highest adventitious shoot induction was obtained on 4.44 mu M of BAP, which gave 6.7 number of shoots after 8 weeks of culture and maximum shoot elongation was observed on WP basal medium without BAP which gave 7.8 cm long shoot 8 weeks after the transfer.