Characteristics of Microorganisms Isolated from Blood Cultures at Nine University Hospitals in Korea during 2009

Abstract

Background: Blood culture is important for determining the etiologic agents of bacteremia and fungemia. Analyses of blood culture results and antimicrobial susceptibility can provide clinicians with relevant information for the empirical treatment of patients. The present study was conducted to assess the frequencies and antimicrobial resistance patterns of clinically important microorganisms from nine hospitals. Methods: Data including microbiological isolates and corresponding antimicrobial susceptibility test results were collected during 2009 from nine and five university hospitals, respectively. Microorganism identification was based on conventional methods. Antimicrobial susceptibility was tested using the VITEK II system or the Clinical and Laboratory Standards Institute disk diffusion method. Results: Of 397,602 blood specimens cultured from nine hospitals, 34,708 (8.7%) were positive for microorganisms. Excluding coagulase-negative Staphylococci (CoNS), Escherichia coli was the most common isolate (13.5%), followed by Staphylococcus aureus (11.5%), Klebsiella pneumoniae (6.5%) and Enterococcus faecium (3.4%). The isolation rate of CoNS was 23.6%, while that of ceftazidime-resistant E. coli showed geographic differences ranging from 11% to 28%. Among the Gram-negative isolates, A.baumannii displayed the highest levels of resistance. The total isolation rate of the Candida species increased compared to the previous reported rate in Korea. Conclusion: Among the isolates, CoNS was the most common, followed by E. coli and S. aureus. The gradual increase in the prevalence of extended-spectrum β-lactamase (ESBL) producers has contributed to the increase in multi-drug resistance among bacterial isolates from bloodstream infections.