가지(Solanum melongena L.) 활성물질의 라디칼 소거능과 산화적 스트레스에 대한 세포 보호 효과

Abstract

To investigate the protective effect of eggplant (Solanum melongena L.) and its active compound, delphinidin,we used in vitro and cellular system. The active fraction from eggplant, BuOH fraction, showed protective effect from hydrogen peroxide-induced oxidative stress in WI-38 fibroblast cells. It suggests that eggplant would have the protective activity from radical-induced oxidative damage and its BuOH fraction would play the crucial role with antioxidative activity. In addition, delphinidin, the active compound from eggplant, exerted the strong 1,1-diphenyl-2-picrylhydrazyl scavenging effect with IC50 value of 6.59 μg/mL. Furthermore, the cellular oxidative stress was induced by 2,2'-azobis (2-amidinopropane)dihydrochloride (AAPH) in LLC-PK1 cells, while treatment of delphinidin atteunated AAPH-induced oxidative stress as dose-dependent manner. The present study suggests the antioxidative activity of eggplant and delphinidin against free radical-induced oxidative stress.