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A RAPD-PCR Method for the Rapid Detection of Bacillus cereus
- Authors
- Lee, Jiyeon; Kwon, Gun-Hee; Park, Jae-Yong; Park, Cheon-Seok; Kwon, Dae Young; Lim, Jinkyu; Kim, Jong Sang; Kim, Jeong Hwan
- Issue Date
- Mar-2011
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Bacillus cereus; food poisoning; rapid detection; RAPD-PCR
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.3, pp 274 - 276
- Pages
- 3
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 21
- Number
- 3
- Start Page
- 274
- End Page
- 276
- ISSN
- 1017-7825
1738-8872
- Abstract
- Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.
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