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큰느타리버섯의 저온적응성 형질에 관련된 SCAR Marker 개발Development of a psychrophilic-SCAR marker for Pleurotus eryngii
- Other Titles
- Development of a psychrophilic-SCAR marker for Pleurotus eryngii
- Issue Date
- 2013
- Publisher
- 한국버섯학회
- Keywords
- OP-S3-1 primer; Pleurotus eryngii; Psychrophilic-SCAR marker
- Citation
- 한국버섯학회지, v.11, no.3, pp 171 - 176
- Pages
- 6
- Indexed
- KCICANDI
- Journal Title
- 한국버섯학회지
- Volume
- 11
- Number
- 3
- Start Page
- 171
- End Page
- 176
- ISSN
- 1738-0294
2288-8853
- Abstract
- Genomic DNAs of psychrophilic strains of Pleurotus eryngii were analyzed by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S3 primers to develop the strain-specific DNA marker. A unique DNA fragment with the size of 480 bp was yielded by OP-S3 primer from the psychrophilic strain. A sequence characterized amplified region (SCAR) marker, designated as OP-S3-1, was designed on the basis of the determined sequence. The PCR analysis with the OP-S3-1 primer showed that this SCAR marker can clearly distinguish the psychrophilic strains from the control strains.
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