Polyphenols isolated from Allium cepa L. induces apoptosis by suppressing IAP-1 through inhibiting PI3K/Akt signaling pathways in human leukemic cells
- Authors
- Han, Min Ho; Lee, Won Sup; Jung, Ji Hyun; Jeong, Jae-Hun; Park, Cheol; Kim, Hye Jung; Kim, GonSup; Jung, Jin-Myung; Kwon, Taeg Kyu; Kim, Gi-Young; Ryu, Chung Ho; Shin, Sung Chul; Hong, Soon Chan; Choi, Yung Hyun
- Issue Date
- Dec-2013
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Allium cepa; Polyphenols; U937; Apoptosis; TRAIL; Akt
- Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.62, pp.382 - 389
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD AND CHEMICAL TOXICOLOGY
- Volume
- 62
- Start Page
- 382
- End Page
- 389
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/20313
- DOI
- 10.1016/j.fct.2013.08.085
- ISSN
- 0278-6915
- Abstract
- Allium cepa Linn is commonly used as supplementary folk remedy for cancer therapy. Evidence suggests that Allium extracts have anti-cancer properties. However, the mechanisms of the anti-cancer activity of A. cepa Linn are not fully elucidated in human cancer cells. In this study, we investigated anti-cancer effects of polyphenols extracted from lyophilized A. cepa Linn (PEAL) in human leukemia cells and their mechanisms. PEAL inhibited cancer cell growth by inducing caspase-dependent apoptosis. The apoptosis was suppressed by caspase 8 and 9 inhibitors. PEAL also up-regulated TNF-related apoptosis-inducing ligand (TRAIL) receptor DR5 and down-regulated survivin and cellular inhibitor of apoptosis 1 (cIAP-1). We confirmed these findings in other leukemic cells (THP-1, 1(562 cells). In addition, PEAL suppressed Akt activity and the PEAL-induced apoptosis was significantly attenuated in Akt-overexpressing U937 cells. In conclusion, our data suggested that PEAL induced caspase-dependent apoptosis in several human leukemic cells including U937 cells. The apoptosis was triggered through extrinsic pathway by up-regulating DR5 modulating as well as through intrinsic pathway by modulating IAP family members. In addition, PEAL induces caspase-dependent apoptosis at least in part through the inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. This study provides evidence that PEAL might be useful for the treatment of leukemia. (C) 2013 Elsevier Ltd. All rights reserved.
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Collections - 농업생명과학대학 > 식품공학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
- College of Medicine > Department of Medicine > Journal Articles
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