큰느타리버섯의 고온적응성 형질에 관련된 SCAR Marker 개발

Abstract

In this study, SCAR marker that differentiates Pleurotus eryngii strains adaptable to high-temperature from controlstrain was developed. Genomic DNAs of 7 control strains of Pleurotus eryngii and 7 Pleurotus eryngii strains adaptable to hightemperaturewere analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). Onehundredtwenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 385 bp wasyielded by OP-A06 primer from the Pleurotus eryngii strains adaptable to high-temperature. A sequence characterized amplifiedregion (SCAR) marker, designated as OP-A06-1-F and OP-A06-1-R, was designed on the basis of the determined sequence. ThePCR analysis with the OP-A06-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strainsadaptable to high-temperature from the control strains.