커피박 첨가가 육계의 소장 효소 활성도, 생화학 지표 및 장내 미생물 균총에 미치는 영향

Abstract

The current study was performed to investigate the effects of dietary supplementation of dried coffee meal (CM)on growth performance, intestinal and blood biochemical index, intestinal enzymes, and cecal microbial populations. A totalof 162, 3-day-old male broiler chicks were randomly allocated into three dietary groups: control group (CON), basal diet addedwith 0.5% CM (CM I), and basal diet added with 1.0% CM (CM II). Dietary supplementation of CM did not change birdperformance and the relative weight of intestinal mucosal tissues. The birds fed the diet supplemented with CM (0.5 and 1.0%)significantly decreased mucosal glucose concentration (P<0.05) without affecting blood glucose level compared with those fedcontrol diet. The level of blood aspartate aminotransferase (AST) significantly increased in CM II group (P<0.05) withoutaffecting γ-glutamyl transpeptidase (γ-GTP) compared with that in the CON group. The specific activity of intestinal maltase,leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) were not affected by dietary supplementation of CM, whereassucrase activity in birds fed the diet supplemented with CM was decreased (P<0.05) compared to that in the control birds. The colony forming units (CFU) of E. coli in the cecum of CM-fed birds was significantly decreased (P<0.05) comparedwith that of control birds without changing the CFU of Lactobacillus. In conclusion, dietary supplementation of lower levelof CM (0.5%) can be used as a beneficial feed resource without liver toxicity in broiler chicks.