Human Umbilical Cord Blood-Derived CD34-Positive Endothelial Progenitor Cells Stimulate Osteoblastic Differentiation of Cultured Human Periosteal-Derived Osteoblasts
- Authors
- Lee, Jin-Ho; Hah, Young-Sool; Cho, Hee-Young; Kim, Jin-Hyun; Oh, Se-Heang; Park, Bong-Wook; Kang, Young-Hoon; Choi, Mun-Jeoung; Shin, Jeong-Kyu; Rho, Gyu-Jin; Jeon, Ryoung-Hoon; Lee, Hee-Chun; Kim, Gyoo-Cheon; Kim, Uk-Kyu; Kim, Jong-Ryoul; Lee, Chang-Il; Byun, June-Ho
- Issue Date
- 1-Mar-2014
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- TISSUE ENGINEERING PART A, v.20, no.5-6, pp.940 - 953
- Indexed
- SCIE
SCOPUS
- Journal Title
- TISSUE ENGINEERING PART A
- Volume
- 20
- Number
- 5-6
- Start Page
- 940
- End Page
- 953
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/19105
- DOI
- 10.1089/ten.tea.2013.0329
- ISSN
- 1937-3341
- Abstract
- The aim of this study was to examine the effects of human umbilical cord blood-derived CD34-positive endothelial progenitor cells (CD34+ EPCs) on osteoblastic differentiation of cultured human periosteal-derived osteoblasts (POs). CD34+ cells from human umbilical cord blood were sorted to purify more EPCs in characterization. These sorted cells showed CD31, VE-cadherin, and KDR expression as well as CD34 expression and formed typical tubes in Matrigel. These sorted cells were referred to as human cord blood-derived CD34+ EPCs. In in vivo bone formation using a miniature pig model, the newly formed bone was clearly examined in defects filled with polydioxanone/pluronic F127 (PDO/Pluronic F127) scaffolds containing either human umbilical cord blood-derived CD34+ EPCs and POs or human umbilical vein endothelial cells (HUVEC) and POs; however, the new bone had the greatest density in the defect treated with CD34+ EPCs and POs. Osteoblastic phenotypes of cultured human POs using ALP activity and von Kossa staining were also more clearly found in CD34+ EPC-conditioned medium than CD34-negative (CD34-) cell-conditioned medium, whereas HUVEC-conditioned medium had an intermediate effect. PCR array for common cytokines and growth factors showed that the secretion of interleukin (IL)-1 beta was significantly higher in CD34+ EPCs than in HUVEC, followed by level in CD34- cells. In addition, IL-1 beta also potently and dose dependently increased ALP activity and mineralization of POs in culture. These results suggest that human umbilical cord blood-derived CD34+ EPCs stimulates osteoblastic differentiation of cultured human POs. The functional role of human umbilical cord blood-derived CD34+ EPCs in increasing the osteogenic phenotypes of cultured human POs may depend on IL-1 beta secreted from human umbilical cord blood-derived CD34+ EPCs.
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