Human Umbilical Cord Blood-Derived CD34-Positive Endothelial Progenitor Cells Stimulate Osteoblastic Differentiation of Cultured Human Periosteal-Derived Osteoblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jin-Ho | - |
dc.contributor.author | Hah, Young-Sool | - |
dc.contributor.author | Cho, Hee-Young | - |
dc.contributor.author | Kim, Jin-Hyun | - |
dc.contributor.author | Oh, Se-Heang | - |
dc.contributor.author | Park, Bong-Wook | - |
dc.contributor.author | Kang, Young-Hoon | - |
dc.contributor.author | Choi, Mun-Jeoung | - |
dc.contributor.author | Shin, Jeong-Kyu | - |
dc.contributor.author | Rho, Gyu-Jin | - |
dc.contributor.author | Jeon, Ryoung-Hoon | - |
dc.contributor.author | Lee, Hee-Chun | - |
dc.contributor.author | Kim, Gyoo-Cheon | - |
dc.contributor.author | Kim, Uk-Kyu | - |
dc.contributor.author | Kim, Jong-Ryoul | - |
dc.contributor.author | Lee, Chang-Il | - |
dc.contributor.author | Byun, June-Ho | - |
dc.date.accessioned | 2022-12-26T23:17:13Z | - |
dc.date.available | 2022-12-26T23:17:13Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2014-03-01 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/19105 | - |
dc.description.abstract | The aim of this study was to examine the effects of human umbilical cord blood-derived CD34-positive endothelial progenitor cells (CD34+ EPCs) on osteoblastic differentiation of cultured human periosteal-derived osteoblasts (POs). CD34+ cells from human umbilical cord blood were sorted to purify more EPCs in characterization. These sorted cells showed CD31, VE-cadherin, and KDR expression as well as CD34 expression and formed typical tubes in Matrigel. These sorted cells were referred to as human cord blood-derived CD34+ EPCs. In in vivo bone formation using a miniature pig model, the newly formed bone was clearly examined in defects filled with polydioxanone/pluronic F127 (PDO/Pluronic F127) scaffolds containing either human umbilical cord blood-derived CD34+ EPCs and POs or human umbilical vein endothelial cells (HUVEC) and POs; however, the new bone had the greatest density in the defect treated with CD34+ EPCs and POs. Osteoblastic phenotypes of cultured human POs using ALP activity and von Kossa staining were also more clearly found in CD34+ EPC-conditioned medium than CD34-negative (CD34-) cell-conditioned medium, whereas HUVEC-conditioned medium had an intermediate effect. PCR array for common cytokines and growth factors showed that the secretion of interleukin (IL)-1 beta was significantly higher in CD34+ EPCs than in HUVEC, followed by level in CD34- cells. In addition, IL-1 beta also potently and dose dependently increased ALP activity and mineralization of POs in culture. These results suggest that human umbilical cord blood-derived CD34+ EPCs stimulates osteoblastic differentiation of cultured human POs. The functional role of human umbilical cord blood-derived CD34+ EPCs in increasing the osteogenic phenotypes of cultured human POs may depend on IL-1 beta secreted from human umbilical cord blood-derived CD34+ EPCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.subject | BONE-MARROW | - |
dc.subject | STEM-CELLS | - |
dc.subject | HEMATOPOIETIC STEM | - |
dc.subject | TNF-ALPHA | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | MINERALIZATION | - |
dc.subject | EXPRESSION | - |
dc.title | Human Umbilical Cord Blood-Derived CD34-Positive Endothelial Progenitor Cells Stimulate Osteoblastic Differentiation of Cultured Human Periosteal-Derived Osteoblasts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Rho, Gyu-Jin | - |
dc.contributor.affiliatedAuthor | Byun, June-Ho | - |
dc.identifier.doi | 10.1089/ten.tea.2013.0329 | - |
dc.identifier.scopusid | 2-s2.0-84896882789 | - |
dc.identifier.wosid | 000332021600007 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART A, v.20, no.5-6, pp.940 - 953 | - |
dc.relation.isPartOf | TISSUE ENGINEERING PART A | - |
dc.citation.title | TISSUE ENGINEERING PART A | - |
dc.citation.volume | 20 | - |
dc.citation.number | 5-6 | - |
dc.citation.startPage | 940 | - |
dc.citation.endPage | 953 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | HEMATOPOIETIC STEM | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | MINERALIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Gyeongsang National University Central Library, 501, Jinju-daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea+82-55-772-0533
COPYRIGHT 2022 GYEONGSANG NATIONAL UNIVERSITY LIBRARY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.