Differential gene-expression profiles from canine cumulus cells of ovulated versus in vitro-matured oocytes
- Authors
- Cho, Su-Jin; Lee, Kyeong-Lim; Kim, Yu-Gon; Kim, Dong-Hoon; Yoo, Jae-Gyu; Yang, Byoung-Chul; Park, Jin-Ki; Kong, Il-Keun
- Issue Date
- 2016
- Publisher
- CSIRO PUBLISHING
- Keywords
- development; dog; in vitro maturation; oogenesis; reproduction
- Citation
- REPRODUCTION FERTILITY AND DEVELOPMENT, v.28, no.3, pp.278 - 285
- Indexed
- SCIE
SCOPUS
- Journal Title
- REPRODUCTION FERTILITY AND DEVELOPMENT
- Volume
- 28
- Number
- 3
- Start Page
- 278
- End Page
- 285
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/16829
- DOI
- 10.1071/RD14086
- ISSN
- 1031-3613
- Abstract
- We compared the nuclear maturation status and gene-expression profiles of canine cumulus cells (CCs) derived from cumulus-oocyte complexes (COCs) that were spontaneously ovulated versus those that were matured in vitro. Cumulus-oocyte complexes were retrieved from uteri by surgical flushing (after spontaneous ovulation) or by ovariectomy follicle aspiration and in vitro maturation. The objective of Experiment 1 was to investigate the nuclear maturation status of in vivo- versus in vitro-matured oocytes. The objective of Experiment 2 was to compare gene-expression profiles of CCs derived from in vivo- versus in vitro-matured COCs. Genes analysed are related to cell maturation, development and apoptosis, including GDF9, MAPK1, PTX3, CX43, Bcl2 and BAX; mRNA expression for all of these genes, except for GDF9, differed (P<0.05) between in vivo- and in vitro-matured CCs. In conclusion, we found that gene-expression profiles are related to the quality of CCs and therefore posit that monitoring gene expression could be a useful strategy to guide attempts to improve in vitro culture systems.
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