Differential gene-expression profiles from canine cumulus cells of ovulated versus in vitro-matured oocytes
DC Field | Value | Language |
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dc.contributor.author | Cho, Su-Jin | - |
dc.contributor.author | Lee, Kyeong-Lim | - |
dc.contributor.author | Kim, Yu-Gon | - |
dc.contributor.author | Kim, Dong-Hoon | - |
dc.contributor.author | Yoo, Jae-Gyu | - |
dc.contributor.author | Yang, Byoung-Chul | - |
dc.contributor.author | Park, Jin-Ki | - |
dc.contributor.author | Kong, Il-Keun | - |
dc.date.accessioned | 2022-12-26T21:23:56Z | - |
dc.date.available | 2022-12-26T21:23:56Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1031-3613 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/16829 | - |
dc.description.abstract | We compared the nuclear maturation status and gene-expression profiles of canine cumulus cells (CCs) derived from cumulus-oocyte complexes (COCs) that were spontaneously ovulated versus those that were matured in vitro. Cumulus-oocyte complexes were retrieved from uteri by surgical flushing (after spontaneous ovulation) or by ovariectomy follicle aspiration and in vitro maturation. The objective of Experiment 1 was to investigate the nuclear maturation status of in vivo- versus in vitro-matured oocytes. The objective of Experiment 2 was to compare gene-expression profiles of CCs derived from in vivo- versus in vitro-matured COCs. Genes analysed are related to cell maturation, development and apoptosis, including GDF9, MAPK1, PTX3, CX43, Bcl2 and BAX; mRNA expression for all of these genes, except for GDF9, differed (P<0.05) between in vivo- and in vitro-matured CCs. In conclusion, we found that gene-expression profiles are related to the quality of CCs and therefore posit that monitoring gene expression could be a useful strategy to guide attempts to improve in vitro culture systems. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | CSIRO PUBLISHING | - |
dc.subject | DEVELOPMENTAL COMPETENCE | - |
dc.subject | MEIOTIC RESUMPTION | - |
dc.subject | GAP-JUNCTIONS | - |
dc.subject | CYTOPLASMIC MATURATION | - |
dc.subject | FOLLICLE DEVELOPMENT | - |
dc.subject | NUCLEAR MATURATION | - |
dc.subject | OVARIAN-FOLLICLE | - |
dc.subject | SECRETED FACTORS | - |
dc.subject | MOUSE OOCYTES | - |
dc.subject | COMPLEX | - |
dc.title | Differential gene-expression profiles from canine cumulus cells of ovulated versus in vitro-matured oocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kong, Il-Keun | - |
dc.identifier.doi | 10.1071/RD14086 | - |
dc.identifier.scopusid | 2-s2.0-84954451413 | - |
dc.identifier.wosid | 000368127000002 | - |
dc.identifier.bibliographicCitation | REPRODUCTION FERTILITY AND DEVELOPMENT, v.28, no.3, pp.278 - 285 | - |
dc.relation.isPartOf | REPRODUCTION FERTILITY AND DEVELOPMENT | - |
dc.citation.title | REPRODUCTION FERTILITY AND DEVELOPMENT | - |
dc.citation.volume | 28 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 278 | - |
dc.citation.endPage | 285 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | DEVELOPMENTAL COMPETENCE | - |
dc.subject.keywordPlus | MEIOTIC RESUMPTION | - |
dc.subject.keywordPlus | GAP-JUNCTIONS | - |
dc.subject.keywordPlus | CYTOPLASMIC MATURATION | - |
dc.subject.keywordPlus | FOLLICLE DEVELOPMENT | - |
dc.subject.keywordPlus | NUCLEAR MATURATION | - |
dc.subject.keywordPlus | OVARIAN-FOLLICLE | - |
dc.subject.keywordPlus | SECRETED FACTORS | - |
dc.subject.keywordPlus | MOUSE OOCYTES | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordAuthor | development | - |
dc.subject.keywordAuthor | dog | - |
dc.subject.keywordAuthor | in vitro maturation | - |
dc.subject.keywordAuthor | oogenesis | - |
dc.subject.keywordAuthor | reproduction | - |
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