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Cited 14 time in webofscience Cited 15 time in scopus
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A rapid and colorimetric loop-mediated isothermal amplification (LAMP) based on HRP-mimicking molecular beacon for the detection of major 6 Listeria species in enoki mushroom

Authors
Lee, Jeong-EunKim, Sol-AMun, HyoyoungKim, Se-RiHa, Kwang-SooShim, Won-Bo
Issue Date
Mar-2022
Publisher
Elsevier BV
Keywords
Colorimetric LAMP assay; Enoki mushroom; Listeria species; Rapid detection; Point-of-care; Molecular diagnostic technology
Citation
Food Control, v.133
Indexed
SCIE
SCOPUS
Journal Title
Food Control
Volume
133
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/1528
DOI
10.1016/j.foodcont.2021.108569
ISSN
0956-7135
1873-7129
Abstract
Since the contamination of Listeria species is considered as a serious problem in public health and food industries, a rapid, accurate, and easy-to-use detection method is highly demanded. The objective of this study is to develop a colorimetric loop-mediated isothermal amplification (cLAMP) assay using a molecular beacon for the rapid and sensitive detection of Listeria species in enoki mushroom. The detection limit of the method optimized at 59.7 degrees C for 6 Listeria species in buffer was all 1 x 10(0) CFU/mL, and the cLAMP was confirmed to be specific to 6 major Listeria species. The period required to complete the cLAMP was within 1 h. The cut-off value of the method for the artificially inoculated mushroom samples was 1 x 10(1) CFU/g. This study suggests that the cLAMP is expected to be used as a point-of-care molecular diagnostic technology because the method does not require any expensive instruments.
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농업생명과학대학 (식품공학부)
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