Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine modelsopen access
- Authors
- Kwon, Ae Jeong; Moon, Ja Young; Kim, Won Kyong; Kim, Suk; Hur, Jin
- Issue Date
- Oct-2016
- Publisher
- JAPAN SOC VET SCI
- Keywords
- antimicrobial peptide; Brucella abortus; brucellosis; vaccination
- Citation
- JOURNAL OF VETERINARY MEDICAL SCIENCE, v.78, no.10, pp.1541 - 1548
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF VETERINARY MEDICAL SCIENCE
- Volume
- 78
- Number
- 10
- Start Page
- 1541
- End Page
- 1548
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/15238
- DOI
- 10.1292/jvms.16-0036
- ISSN
- 0916-7250
- Abstract
- Brucella abortus cells were lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36). Next, the protection efficacy of the lysed fragment as a vaccine candidate was evaluated. Group A mice were immunized with sterile PBS, group B mice were intraperitoneally (ip) immunized with 3 x 10(8) colony-forming units (CFUs) of B. abortus strain RB51, group C mice were immunized ip with 3 x 10(8) cells of the B. abortus vaccine candidate, and group D mice were orally immunized with 3 x 10(9) cells of the B. abortus vaccine candidate. Brucella lipopolysaccharide (LPS)-specific serum IgG titers were considerably higher in groups C and D than in group A. The levels of interleukin (IL)-4, IL-10, tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) were significantly higher in groups B-D than in group A. After an ip challenge with B. abortus 544, only group C mice showed a significant level of protection as compared to group A. Overall, these results show that ip immunization with a vaccine candidate lysed by GI24 can effectively protect mice from systemic infection with virulent B. abortus.
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