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Properties of antimicrobial substances produced by Bacillus species isolated from rice straw

Authors
Liu, X.Yao, Z.Shim, J.M.Lee, K.W.Kim, H.-J.Kim, J.H.
Issue Date
2017
Publisher
Korean Society for Microbiolog and Biotechnology
Keywords
Antimicrobials; Bacillus amyloliquefaciens; Bacillus licheniformis; Lipopeptides
Citation
Korean Journal of Microbiology and Biotechnology, v.45, no.2, pp 133 - 142
Pages
10
Indexed
SCOPUS
Journal Title
Korean Journal of Microbiology and Biotechnology
Volume
45
Number
2
Start Page
133
End Page
142
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/14933
DOI
10.4014/mbl.1611.11004
ISSN
1598-642X
Abstract
Two hundred bacilli were isolated from rice straw, and 3 strains showing strong inhibitory activities against Bacillus cereus ATCC14579 were selected for further analyses. RSC15 was identified as B. licheni-forms, and RSC26 and RSC42 were identified as B. amyloliquefaciens by molecular identification methods. The inhibitory activities were heat stable, and half of the activity was retained for 20 min at 100 °C. SDS-PAGE analyses of the culture supernatant indicated that 2 different kinds of antibacterial substances were present with sizes below 3.5 kDa. Antibacterial substances produced by B. licheniformis RSC15 were partially purified by column chromatography, and the specific activity increased from 955.0 AU/mg to 6,400 AU/mg. ? 2017, The Korean Society for Microbiology and Biotechnology.
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