차씨오일의 항산화 활성 및 뇌 해마유래 HT22 세포에서의 산화적 스트레스에 대한 보호효과

Abstract

The antioxidant activities and hippocampal neuroprotective effects of tea (Camellia sinensis) seed oil against H2O2-induced oxidative stress were investigated to confirm its physiological effects. The antioxidant effects of tea seed oil were evaluated by measuring its 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging activities and malondialdehyde inhibitory effect. Tea seed oil was found to have significant antioxidant activity and inhibitory effect of lipid peroxidation. To confirm the ameliorating effect of the cholinergic system, the inhibitory effect of acetylcholinesterase (AChE) derived from PC12 was investigated. Tea seed oil was found to inhibit the AChE activity. Also, the protective effects of hippocampal neuronal cells against H2O2-induced neuronal cytotoxicity were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and 2',7'-dichlor-ofluorescein diacetate in HT22 cells. Tea seed oil was found to significantly increase the cell viability and reactive oxygen species scavenging activity.