Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infectionImmunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection
- Other Titles
- Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection
- Authors
- Lauren Togonon Arayan; Tran Xuan Ngoc Huy; Alisha Wehdnesday Bernardo Reyes; HUYNH TAN HOP; Vu Hai Son; 민원기; 이후장; 김석
- Issue Date
- 2018
- Publisher
- 한국예방수의학회
- Keywords
- B. abortus; recombinant proteins; vaccine; cytokine; protection
- Citation
- 예방수의학회지, v.42, no.4, pp.157 - 162
- Indexed
- KCI
- Journal Title
- 예방수의학회지
- Volume
- 42
- Number
- 4
- Start Page
- 157
- End Page
- 162
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/12377
- DOI
- 10.13041/jpvm.2018.42.4.157
- ISSN
- 2287-7991
- Abstract
- This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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