Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lauren Togonon Arayan | - |
dc.contributor.author | Tran Xuan Ngoc Huy | - |
dc.contributor.author | Alisha Wehdnesday Bernardo Reyes | - |
dc.contributor.author | HUYNH TAN HOP | - |
dc.contributor.author | Vu Hai Son | - |
dc.contributor.author | 민원기 | - |
dc.contributor.author | 이후장 | - |
dc.contributor.author | 김석 | - |
dc.date.accessioned | 2022-12-26T17:32:48Z | - |
dc.date.available | 2022-12-26T17:32:48Z | - |
dc.date.created | 2022-12-14 | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 2287-7991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/12377 | - |
dc.description.abstract | This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국예방수의학회 | - |
dc.title | Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection | - |
dc.title.alternative | Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 김석 | - |
dc.identifier.doi | 10.13041/jpvm.2018.42.4.157 | - |
dc.identifier.bibliographicCitation | 예방수의학회지, v.42, no.4, pp.157 - 162 | - |
dc.relation.isPartOf | 예방수의학회지 | - |
dc.citation.title | 예방수의학회지 | - |
dc.citation.volume | 42 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 157 | - |
dc.citation.endPage | 162 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART002426339 | - |
dc.description.journalClass | 2 | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | B. abortus | - |
dc.subject.keywordAuthor | recombinant proteins | - |
dc.subject.keywordAuthor | vaccine | - |
dc.subject.keywordAuthor | cytokine | - |
dc.subject.keywordAuthor | protection | - |
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