Identification of Proteins Differentially Expressed by Quercetin Treatment in a Middle Cerebral Artery Occlusion Model: A Proteomics Approach
- Authors
- Shah, Fawad-Ali; Park, Dong-Ju; Koh, Phil-Ok
- Issue Date
- Aug-2018
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- Middle cerebral artery occlusion; Proteomics; Quercetin; Stroke
- Citation
- NEUROCHEMICAL RESEARCH, v.43, no.8, pp.1608 - 1623
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEUROCHEMICAL RESEARCH
- Volume
- 43
- Number
- 8
- Start Page
- 1608
- End Page
- 1623
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/11431
- DOI
- 10.1007/s11064-018-2576-x
- ISSN
- 0364-3190
- Abstract
- Cerebral ischemia is a major cause of death and neurological disability. It also leads to severe brain tissue damage by excessive generation of oxidative stress. Quercetin is a bioflavonoid substance that acts an antioxidant agent and exerts a neuroprotective effect against cerebral ischemia. The aim of this study was to detect specific proteins that are differentially expressed in response to quercetin treatment in focal cerebral ischemia. Adult male rats were intraperitoneally injected with vehicle or quercetin (10 mg/kg) 30 min prior to right middle cerebral artery occlusion (MCAO). Brain tissues were collected 24 h after MCAO surgery and right cerebral cortices proteins were identified by two-dimensional gel electrophoresis and mass spectrometry. MCAO leads to neurological behavior disorders, infarction, and histopathological change. However, quercetin treatment alleviated MCAO-induced neuronal deficits and damages. We identified specific proteins differentially expressed between vehicle- and quercetin-treated animals. Among these detected proteins, isocitrate dehydrogenase [NAD(+)], adenosylhomocysteinase, pyruvate kinase, and ubiquitin carboxy terminal hydrolase L1 were decreased in vehicle-treated animals, while quercetin administration alleviated the MCAO-induced decreases in these proteins. However, 60 kDa heat shock protein and collapsin response mediator protein 2 were increased in the vehicle-treated animals, and quercetin treatment attenuated increases in these proteins. The expression changes in these proteins were confirmed by Western blot and reverse transcription-PCR analyses. These proteins are associated with cellular differentiation, metabolism, and oxidative stress related proteins. These results suggest that quercetin reduces ischemic injury by modulating the expression of various proteins in focal cerebral ischemia.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.gnu.ac.kr/handle/sw.gnu/11431)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.