Molecular weight-dependent bioactive potential of chicken feet collagen hydrolysates derived from Abelmoschus manihot-fed broilers

초록

This study investigated the impact of supplementing broiler diets with 0, 0.5, or 1% Abelmoschus manihot powder on the extraction yield of chicken feet collagen and the bioactivities of the resulting collagen hydrolysates, with emphasis on antioxidant capacity (assessed by DPPH, ABTS, and Fe2+-chelating assays) and inhibition of nitric oxide (NO) production in H2O2-stimulated RAW 264.7 macrophages. Chicken feet collagen was extracted using citric acid, achieving yields between 14.8 and 19.0%, and subsequently hydrolyzed using collagenase D for either 3 or 6 h, followed by fractionation into <3, 3-10, and >10 kDa fractions. All molecular weight fractions displayed substantial Fe2+-chelating capabilities. The <3 kDa fraction demonstrated the greatest inhibition of NO production, whereas the >10 kDa fraction exhibited the highest ABTS radical scavenging activity. Dietary supplementation with Abelmoschus manihot powder did not significantly alter collagen yield or overall antioxidant activity of the hydrolysates, indicating that the biofunctional properties were closely associated with molecular weight distribution than dietary intervention under normal physiological conditions. Overall, these findings indicate that molecular weight fractionation is a key determinant of the bioactive potential of chicken feet-derived collagen hydrolysates, showing differences across molecular weight fractions in antioxidant capacity and NO inhibition. These results provide baseline information for evaluating feed-related effects on poultry by-products.

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