Induction of the cell cycle arrest and apoptosis by flavonoids isolated from Korean Citrus aurantium L. in non-small-cell lung cancer cells

Park, Kwang Il; Park, Hyeon Soo; Nagappan, Arulkumar; Hong, Gyeong Eun; Lee, Do Hoon; Kang, Sang Rim; Kim, Jin A.; Zhang, Jue; Kim, Eun Hee; Lee, Won Sup; Shin, Sung Chul; Hah, Young Sool; Kim, Gon Sup

doi:10.1016/j.foodchem.2012.06.097

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Induction of the cell cycle arrest and apoptosis by flavonoids isolated from Korean Citrus aurantium L. in non-small-cell lung cancer cells

Park, Kwang Il;
Park, Hyeon Soo;
Nagappan, Arulkumar;
Hong, Gyeong Eun;
Lee, Do Hoon;
... Lee, Won Sup;
... Kim, Gon Sup;
외 6명

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초록

This study investigated the anti-proliferative and apoptotic effect of flavonoids isolated from Korean Citrus aurantium L using A549 lung cancer cells. Flavonoids potently inhibited of A549 cells in a dose-dependent manner, whereas flavonoids had a weak inhibitory effect on proliferation of WI-38 cells. Flow cytometry and Western blot analysis showed that flavonoids induced cell cycle arrest at the G2/M check-point by controlling the proteins expression level of cyclin B1, cdc2, cdc25c and p21(WAF1/CIP1). Also, flavonoids induced apoptosis through the regulation of the expression of caspases, cleaved PARP and Bax/Bcl-xL ratio. The activity of caspase-3 on A549 cells increased in a dose-dependent manner. These results clearly indicated that the anti-cancer effect of flavonoids on A549 cells follows multiple cellular pathways through G2/M arrest and the induction of apoptosis. (C) 2012 Elsevier Ltd. All rights reserved.

키워드

Korean Citrus aurantium L.; Flavonoid; Human lung cancer cells; Cell cycle arrest; Apoptosis; BCL-2 FAMILY PROTEINS; HUMAN COLON-CANCER; MAMMALIAN-CELLS; PHOSPHORYLATION; KINASE; TRANSITIONS; ACTIVATION; REGULATORS; PATHWAY; DEATH

제목: Induction of the cell cycle arrest and apoptosis by flavonoids isolated from Korean Citrus aurantium L. in non-small-cell lung cancer cells

저자: Park, Kwang Il; Park, Hyeon Soo; Nagappan, Arulkumar; Hong, Gyeong Eun; Lee, Do Hoon; Kang, Sang Rim; Kim, Jin A.; Zhang, Jue; Kim, Eun Hee; Lee, Won Sup; Shin, Sung Chul; Hah, Young Sool; Kim, Gon Sup

DOI: 10.1016/j.foodchem.2012.06.097

발행일: 2012-12-15

유형: Article

저널명: Food Chemistry

권: 135

호: 4

페이지: 2728 ~ 2735