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Induction of the cell cycle arrest and apoptosis by flavonoids isolated from Korean Citrus aurantium L. in non-small-cell lung cancer cells
- Park, Kwang Il;
- Park, Hyeon Soo;
- Nagappan, Arulkumar;
- Hong, Gyeong Eun;
- Lee, Do Hoon;
- ... Lee, Won Sup;
- 외 7명
WEB OF SCIENCE
47SCOPUS
63초록
This study investigated the anti-proliferative and apoptotic effect of flavonoids isolated from Korean Citrus aurantium L using A549 lung cancer cells. Flavonoids potently inhibited of A549 cells in a dose-dependent manner, whereas flavonoids had a weak inhibitory effect on proliferation of WI-38 cells. Flow cytometry and Western blot analysis showed that flavonoids induced cell cycle arrest at the G2/M check-point by controlling the proteins expression level of cyclin B1, cdc2, cdc25c and p21(WAF1/CIP1). Also, flavonoids induced apoptosis through the regulation of the expression of caspases, cleaved PARP and Bax/Bcl-xL ratio. The activity of caspase-3 on A549 cells increased in a dose-dependent manner. These results clearly indicated that the anti-cancer effect of flavonoids on A549 cells follows multiple cellular pathways through G2/M arrest and the induction of apoptosis. (C) 2012 Elsevier Ltd. All rights reserved.
키워드
- 제목
- Induction of the cell cycle arrest and apoptosis by flavonoids isolated from Korean Citrus aurantium L. in non-small-cell lung cancer cells
- 저자
- Park, Kwang Il; Park, Hyeon Soo; Nagappan, Arulkumar; Hong, Gyeong Eun; Lee, Do Hoon; Kang, Sang Rim; Kim, Jin A.; Zhang, Jue; Kim, Eun Hee; Lee, Won Sup; Shin, Sung Chul; Hah, Young Sool; Kim, Gon Sup
- 발행일
- 2012-12-15
- 유형
- Article
- 저널명
- Food Chemistry
- 권
- 135
- 호
- 4
- 페이지
- 2728 ~ 2735
- 언어
- ENG
- 출판사
- ELSEVIER SCI LTD
- 발행국가
- 영국
- 분량
- 8 페이지
- ISSN
- E 1873-7072
P 0308-8146