Protective effect of Platycodon grandiflorus extract on LPS-induced cytotoxicity against HT-29 cells

초록

This study investigated the prebiotic-like activity and cytoprotective effects of a water extract from Platycodon grandiflorus (WPG) on intestinal epithelial HT-29 cells. The WPG significantly affected the viability and titratable acidity of Limosilactobacillus reuteri and Levilactobacillus brevis. It increased cell viability and suppressed oxidative stress against LPS-induced cytotoxicity, thereby exerting protective effects on intestinal epithelial HT-29 cells. Furthermore, WPG downregulated the expression of inflammatory mediators, including TLR-4, MyD88, p-NF-κB, and iNOS, upregulated tight junction proteins, such as ZO-1, occludin, and claudin-1, and significantly modulated the expression of apoptotic proteins, including p-AKT, p-JNK, BAX, and Bcl-2. The major bioactive compound in WPG was identified as platycodin D by highperformance liquid chromatography.

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