Characterization of gene expression profiles and functional analysis of peptidoglycan recognition protein 2 from rock bream (Oplegnathus fasciatus)
- Choi, Kwang-Min; Joo, Min-Soo; Cho, Dong Hee; Bae, Jin-Sol; Jung, Ji-Min; Hwang, Jee Youn; Kwon, Mun-Gyeong; Seo, Jung Soo; Hwang, Seong Don; Jee, Bo-Yeong; Kim, Do-Hyung; Park, Chan-Il
- Issue Date
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Streptococcus iniae; Edwardsiella piscicida; Red sea bream iridovirus (RSIV); Peptidoglycan recognition protein 2; Oplegnathus fasciatus
- FISH & SHELLFISH IMMUNOLOGY, v.84, pp.1068 - 1074
- Journal Title
- FISH & SHELLFISH IMMUNOLOGY
- Start Page
- End Page
- Peptidoglycan recognition protein 2 (PGRP2) is a Zn2+-dependent peptidase that plays important roles in binding to microbial components of the cell membrane, inducing phagocytosis and antimicrobial activity. Rock bream (Oplegnathus fasciams) PGRP2 (RbPGRP2) was identified in the intestine by next generation sequencing (NGS) analysis. The open reading frame (ORF) the RbPGRP2 cDNA (470 amino acid residues) contains a peptidoglycan recognition protein domain (residues 300 to 446). Alignment analysis revealed that RbPGRP2 shares 37.6-53.5% overall sequence identity with the PGRP2s of other species. Phylogenetic analysis revealed that RbPGRP2 clustered together with PGRP2s from teleosts. In healthy rock bream, RbPGRP2 was found to be ubiquitously expressed in all of the examined tissues, especially in the liver. RbPGRP2 expression was significantly upregulated in all of the examined tissues of rock bream after infection with Edwardsiella piscicida, Streptococcus iniae and red sea bream iridovirus (RSIV) compared with the control. Purified rRbPGRP2 interactions with bacteria and inhibited the growth of bacteria in the presence of Zn2+. These results indicate that RbPGRP2 plays an important role in the innate immune response against bacterial infection.
- Files in This Item
- There are no files associated with this item.
- Appears in
- 해양과학대학 > 양식생명과학과 > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.