Rotenoisin A is a novel anti-adipogenic compound
- Authors
- Cho, Hang-Hee; Park, Hyeon Soo; Jang, Sun-Hee; Won, Chungkil; Kim, Hong-Duck; Kim, Tae Hoon; Cho, Jae-Hyeon
- Issue Date
- 1-Jan-2019
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Rotenoisin A; Antiadipogenic effect; Transcription factors; AMPK
- Citation
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.29, no.1, pp.89 - 96
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- Volume
- 29
- Number
- 1
- Start Page
- 89
- End Page
- 96
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/9546
- DOI
- 10.1016/j.bmcl.2018.11.008
- ISSN
- 0960-894X
- Abstract
- The purpose of this study was to investigate the mechanisms underlying the inhibitory effects of rotenoisin A on adipogenesis in 3T3-L1 preadipocytes. 3T3-L1 cells were treated with rotenoisin A for 8 days after the induction of differentiation. Oil-red O staining showed that rotenoisin A significantly inhibited DMI-induced lipid accumulation and adipocyte differentiation. We found that rotenoisin A treatment of 3T3-L1 preadipocytes significantly reduced the mRNA and protein levels of the key adipocyte-specific transcription factors C/EBP beta, C/EBP alpha, and PPAR gamma and markedly inhibited the expression of fatty acid-binding protein (aP2), fatty acid synthase (FAS), and lipoprotein lipase (LPL). Furthermore, we observed that rotenoisin A substantially increased the phosphorylation of AMP-activated protein kinase (AMPK) and its downstream target phosphorylated acetyl CoA carboxylase (ACC). However, co-treatment with Compound C, an AMPK inhibitor, reversed the rotenoisin A-induced inhibition of the expression of the adipogenic transcription factors C/EBP alpha and PPAR gamma and decreased the levels of phosphorylated AMPK in differentiated 3T3-L1 cells. These results demonstrated that the anti-adipogenesis mechanism involves the down-regulation of critical adipogenic transcription factors, including C/EBP beta, C/EBP alpha, and PPAR gamma, through activation of the AMPK signaling pathway by rotenoisin A.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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